Immunology RSM

BIOL20952 Immunology RSM 10 Semester 2 Undergraduate Level 2 Kathleen Nolan Unit coordinator School of Biological Sciences 1 FHEQ level (Framework for Higher Education Qualifications) ' Middle part of Bachelors ' European Credit Transfer & Accumulation System Rating : 5.0 During this Unit you will apply commonly used immune-based techniques in research-based scenarios to investigate immune responses in different situations of infection and disease. The content of the unit is designed to reinforce and stimulate interrogation of material in the co-requisite lecture unit BIOL21242 Immunology. During this Unit you will apply commonly used immune-based techniques in research-based scenarios to investigate the nature of immune responses in different situations of infection and disease. The Unit is organised as four stand-alone weeks and has been designed to reinforce and stimulate interrogation of material in the co-requisite BIOL21242 Immunology lecture Unit. In week 1 (lab-based) you will use histology, H&E staining and immune fluorescent-based analysis of tissue sections from infected and non-infected, normal and genetically modified transgenic mice to investigate how inflammation during infection can cause tissue pathology. In week 2 (PC cluster-based) you will take an historical journey to revisit key experiments and use nucleotide/protein sequence and genomic and protein structure analysis to explore the nature of antibody variability and binding function. In week 3 (lab-based) you will use ELISA to investigate the nature of what constitutes a protective response in mice infected with the murine whip worm Trichursi muris. In the final week, (Lab- & classroom-based), you will stain cells from mouse spleen during an introduction to the methodology and processes of flow cytometry, including an introduction to the process of acquisition and interpretation of data. You will visit the FBMH flow facility to see your samples analysed and data collected, and you will complete a problem set that requires you to interpret your own and other flow cytometry data to draw conclusions in different immune-based clinical scenarios.  <ul><li>To expose students to the theory and practice of a selection of immune-based techniques commonly used in broad research and clinical laboratory settings</li><li>To apply these techniques in question-based experimental settings that illustrate core immune principles</li><li>To develop practical, organisational and team working skills within a research environment</li><li>To enhance ability in experimental design, critical interpretation and presentation of data, and reporting of experimental findings   </li></ul> By the end of this unit you should be able to;  • follow instruction to work safely and effectively with others in a research laboratory setting  • apply knowledge of principles underlying the experimental methods used to obtain and record meaningful data  • document experiments in a counter-signed lab book in accordance with good laboratory practice  • critically consider appropriateness of controls used and the validity and presentation of results generated to inform on the research question being asked  • draw on knowledge from other areas of your learning to interpret results and inform on the nature of the immune response in different infection and disease scenarios  • interpret similar data presented in the form of archived data or case studies  • identify variable and hypervariable regions in linear sequences and in three-dimensional space in an intact antibody and comment on this in relation to generation of variability and antibody binding functions  • appreciate other situations in which the research techniques experienced could be informative    Group/team working Group collaboration and problem solving during experimental work. Research Exposer to the theory and practice of immune-based techniques commonly used in broad research and clinical laboratory settings; engaging with primary literature; analysing and discussing scientific concepts and results to investigate, solve problems and draw conclusions from results obtained. Written communication Documenting experiments in a lab book; written reporting of observations and findings for in lab assessment and full-practical write up; discussion on the online discussion forum. • Practical work will be performed in small groups for collaborative planning and experimental management.  • Online support material will be provided through Canvas.  • Problem-based learning and assessment will be provided in class as open book exercises. 0 Other 48% 3 Report 50% 5 Portfolio 2% Written report   Full write-up of an assigned practical investigation in the style of journal paper (5 pages+1 page for figures) (worth 50% of the unit mark).Portfolio  Lab book (worth 2% of the unit mark): recording of experimental method and data acquisition in a dated lab book with page numbering and indexing, to be signed-off by demonstrators/staff at the end of each relevant experimental period.  Other  Short answer and data interpretation problems performed within the scheduled sessions (worth 48% of the unit mark)  <ul><li>In-class verbal feedback throughout</li><li>Online ’Community Discussion Forum’ on Canvas;anonymous submission and discussion</li><li>Written feedback for summative short answer and data interpretation assignments  </li><li>Written feedback for the summative full-report write-up  </li></ul> BIOL21252 Parasitology Co-Requisite Recommended BIOL21242 Immunology Co-Requisite Compulsory BIOL20942 Co-requisite is BIOL21242 BSc(Hons) Biology BSc(Hons) Biology Second Year Optional BSc (Hons) Biology with IE/PE BSc (Hons) Biology with IE/PE Second Year Optional BSc(Hons) Biology w Mod Lang BSc(Hons) Biology w Mod Lang Second Year Optional BSc(Hons) Biology w Mod Lang BSc(Hons) Biology w ML (Asian) Second Year Optional BSc (Hons) Biomed Sci w IE/PE BSc (Hons) Biomed Sci w IE/PE Second Year Optional BSc(Hons) Biomedical Sciences BSc(Hons) Biomedical Sciences Second Year Optional BSc(Hons) Biomed Sci w Mod Lan BSc(Hons) Biomed Sci w Mod Lan Second Year Optional BSc(Hons) Biomed Sci w Mod Lan BSc(Hons) Bio Sci w ML (Asian) Second Year Optional MSci Biology MSci Biology Second Year Optional MSci Biomedical Sciences MSci Biomedical Sciences Second Year Optional MSci Immunology MSci Immunology Second Year Mandatory BSc(Hons) Immunology BSc(Hons) Immunology Second Year Mandatory BSc (Hons) Immunology w/ModLan BSc (Hons) Immunology w/ModLan Second Year Mandatory BSc (Hons) Immunology w/ModLan BSc (Hons) Immuno w ML (Asian) Second Year Mandatory BSc (Hons) Immunology w/IE/PE BSc (Hons) Immunology w/IE/PE Second Year Mandatory BSc(Hons) Biol with Entrepren BSc(Hons) Biol with Entrepren Second Year Optional BSc(Hons) Biomed Sci W Entrepr BSc(Hons) Biomed Sci w Entre Second Year Optional BSc(Hons) Immunol w Entre BSc(Hons) Immunol w Entre Second Year Mandatory N Compulsory:<ul><li>Review BIOL21242 lecture material, in particular, ‘Recognition of antigen by B cells and antibodies’, ‘The genetics of antigen receptors I: B cell receptor diversity’ and ‘Generation of antibodies and monoclonal antibodies’</li></ul>Recommended:<ul><li>Kuby Immunology (8th edition, 2018), written by Stranford, Owen, Jones and Punt (MacMillan Education)</li><li>Janeway’s Immunobiology (10th edition, 2022), written by Kenneth Murphy and Casey Weaver (Garland Science)</li><li>Roitt’s Essential Immunology (13th edition, 2017), written by Delves, Martin, Burton and Roitt (Wiley-Blackwell).  </li></ul>Reference:  <ul><li>Hurst RJ and Else KJ (2013). Trichuris muris research revisited: a journey through time. Parasitology, 140(11):1325-39 </li></ul> Practical classes & workshops 72 0 28 RSM Attendance guidelines: Students must attend all scheduled RSM sessions on time (N.B. Health and safety information is often delivered at the start of practical sessions, and consequently students who are not present at the start may be asked to leave the lab).