Disease Modelling and Genome Engineering

BIOL67672 Disease Modelling and Genome Engineering 15 Semester 2 Postgraduate Taught Level 7 Samina Naseeb Unit coordinator School of Biological Sciences 1 FHEQ level (Framework for Higher Education Qualifications) ' Masters/Integrated Masters P4 ' European Credit Transfer & Accumulation System Rating : 7.5 This unit will provide essential and contemporary knowledge on the importance of using model systems to investigate the functional genomics of inherited human diseases. The unit will also review genomic engineering technologies and provide a workshop for the  in silico design of CRISPR-Cas9 reagents.  Students will be provided with a number of example engineered models to study human disease and will be able to understand the merits and drawbacks of many of the cell and animal models available to researchers. The ethics of using animal models and the potential of genomic engineering to alter human genomes will also be discussed. The unit is led and delivered by academics with a wealth of experience in using a wide range of models to study human genetic disease.   <div class="OutlineElement Ltr SCXW23691226 BCX8" style="-webkit-tap-highlight-color:transparent;-webkit-text-stroke-width:0px;-webkit-user-drag:none;background-color:rgb(255, 255, 255);clear:both;color:rgb(0, 0, 0);cursor:text;direction:ltr;font-family:"Segoe UI", "Segoe UI Web", Arial, Verdana, sans-serif;font-size:12px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin:0px;orphans:2;overflow:visible;padding:0px;position:relative;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;user-select:text;white-space:normal;widows:2;word-spacing:0px;">This unit will provide essential and contemporary knowledge on the importance of using model systems to investigate the functional genomics of inherited human diseases. The unit will also review genomic engineering technologies and provide a workshop for the design of CRISPR-Cas9 reagents.  Students will be provided with a number of example engineered models to study human disease and will be able to understand the merits and drawbacks of many of the cell and animal models available to researchers. The ethics of using animal models and the potential of genomic engineering to alter human genomes will also be discussed. The unit is led and delivered by academics with a wealth of experience in using a wide range of models to study human genetic disease.  </div> <ul> <li>Provide an understanding of why animal and cellular models are necessary for studying the functional genomics of human disease.</li> <li>Provide an understanding of the different model systems available for studying human disease.</li> <li>Provide an understanding of how to manipulate gene expression in a variety of model systems.</li> <li>Provide an understanding of the genome engineering techniques available, how to design associated reagents, and how to use them.</li> <li>Provide an understanding of the ethical considerations associated with animal models and genome engineering.</li> <li>Be able to use literature and online resources to access information on disease modelling and genomic engineering.</li> <li>Be able to apply knowledge of model systems and genome engineering to critically analyse published data and to design experiments.</li></ul> <ul> <li> Use advanced knowledge in how to manipulate the genomic sequence and gene expression of cell and animal models to evaluate models of human disease.</li> <li> Use advanced knowledge in how to manipulate the genomic sequence and gene expression of cells, animals and humans to evaluate therapeutic treatments for inherited human disease.</li> <li> Describe and critically evaluate a range of contemporary genomic technologies to alter the sequence and expression of genes.</li> <li> Debate the ethical arguments about the use of animal models to study human disease and the ethical concerns about being able to alter genomes in vitro and in vivo.</li></ul> <ul> <li> Critically evaluate the methods and technologies used to generate model systems of inherited human diseases.</li> <li> Critically evaluate the methods and technologies used to generate therapies employing altered gene expression for the treatment of inherited human diseases.</li></ul> Review and critically analyse the scientific literature relevant to model systems and genomic engineering pertinent to inherited human disease. Present a topic from the literature on a model system, or an application of a technology used in disease modelling or treatment of an inherited human disease. Use web-based tools to design regents to alter the expression of genes or genomic sequence. Analytical skills Analysis of complex data Group/team working Collaborative skills through group working Oral communication Oral written presentation skills Problem solving Problem solving skills Written communication Scientific writing This module will be delivered over a one week period and consists of a series of face to face lectures, interactive seminars, and computer practicals. Lectures are supported with online resources and/or key references. 1 Written exam 80% 7 Oral assessment/presentation 20% 2 hour written examination comprising of two parts [MCQs (30%) and essay (50%)] in the semester 2 examination period (80%).  Written Feedback with 15 working days MSc Biotechnology Enterprise MSc Biotechnology Enterprise PGDT Taught Component Optional MSc Genomic Medicine FT MSc Genomic Medicine FT PGDT Taught Component Optional MSc Genomic Medicine FT MSc Genomic Medicine FT (HEE) PGDT Taught Component Optional MSc Genomic Medicine PT MSc Genomic Medicine PT (HEE) PGDT Taught Component Optional PG Diploma Genomic Medicine FT PG Diploma Genomic Medicine FT PGDT Taught Component Optional PG Diploma Genomic Medicine FT PGDip Genomic Medicine FT(HEE) PGDT Taught Component Optional PG Diploma Genomic Medicine PT PG Diploma Genomic Medicine PT PGDT Taught Component Optional PG Diploma Genomic Medicine PT PGDip Genomic Medicine PT(HEE) PGDT Taught Component Optional PG Cert Genomic Medicine FT PG Cert Genomic Medicine FT PGDT Taught Component Optional PG Cert Genomic Medicine FT PGCert GenomicMedicine FT(HEE) PGDT Taught Component Optional PG Cert Genomic Medicine PT PG Cert Genomic Medicine PT PGDT Taught Component Optional PG Cert Genomic Medicine PT PG Cert Genomic Med PT (HEE) PGDT Taught Component Optional Genomic Medicine - CPD Genomic Medicine - CPD PGDT Taught Component Optional Genomic Medicine - CPD Genomic Medicine - CPD (HEE) PGDT Taught Component Optional Y The Unit’s Canvas page provides a selection of sources as in introduction to model systems and CRISPR. As many CRISPR overviews are similar it is up to the student to determine which one they prefer. It is not intended that the student reads them all. There are also articles on CRISPR in biotechnology which may be of interest to the Enterprise and Biotechnology students. The 'Gene Drive' folder contains references to accompany Matthew Cobb's lecture on Gene Drives.Much of the content is for interest. The aim is that students should be able to follow the unit with only a limited background of genomic engineering.  0 0 150