CHEM63003
Mass Spectrometry
20
Variable teaching patterns
Postgraduate Taught
Level 7
Department of Chemistry
1
FHEQ level (Framework for Higher Education Qualifications) ' Masters/Integrated Masters P4 '
European Credit Transfer & Accumulation System Rating : 10.0
<ul> <li> Instrumentation: Interfaces to mass spectrometers (how do we get the sample into the instrument), ionization techniques (how do we ionise the sample), mass analyzers (how do we determine the mass to charge ratio), ion detection (how we detect a signal), and data acquisition (how we obtain the data).</li> <li> The basic principles of the mass analysers will be taught with reference to SIMION – where in the student will be able to understand the method of mass analysis, and the influence of supplied voItages and pressure on the acquired spectrum. </li> <li> Interpretation of the mass spectrum will be taught with reference to examples from chemical and biological molecules. The material will cover the interpretation of parent and fragment ion data, isotopes, charge state distributions and mass measurement. General instrument operation.</li> <li> Facilities of the mass spectrometer: ion fragmentation, ion activation, collisionally activated dissociation (CAD) Electron transfer/capture dissociation, photo-dissociation tandem mass spectrometry (MS/MS), modified and emerging techniques.</li> <li> Hyphenation of LC and GC to mass spectrometers; complex mixture analysis</li></ul>
<p>1. Teach mass spectrometry and how it is applied to chemical and biological analysis.</p><p>2. Equip the students taking it with a practical and theoretical knowledge of the components of common commercially available mass spectrometers. </p><p>3. Introduce the components of a mass spectrometer and how it can be used to separate and identify compounds. </p><p>4. Explain the common methods of ionisation and to highlight choice of ionisation to suit the compound(s) to be analysed. </p><p>5. Explain the major types of mass analyser – and why they might be used. </p><p>6. Consider the rationale for the use of hyphenated mass spectrometry such as LC-MS..</p><p>7. Develop an understanding of the basic principles of tandem mass spectrometry including fragmentation methods and mechanisms. </p><p>8. Develop the skills required to interpret spectra and to apply this to biological and chemical samples. </p>
<div> • describe and explain the principles of operation of common mass analysers</div><div> • explain the major methods of ionisation and their applications</div><div> • describe the principles underlying ion detection and data acquisition</div><div> • explain the principles and applications of tandem mass spectrometry</div><div> • explain the principles of hyphenated chromatographymass spectrometry and the experimental requirements for coupling chromatography to mass spectrometers</div><div> • compare and contrast examples of major commercially available mass spectrometers</div>
<ul> <li> apply the principles of mass analysis and the program SIMION to explain mass spectrometric processes </li> <li> perform systematic interpretation of mass spectrometry data and perform structural analysis using that data </li> <li> select the optimum ionisation method for analysis for a range of chemical compounds and biological mixtures</li> <li> deconstruct and critique descriptions of mass spectrometry analysis in published work and technical reports </li> <li> solve a range of unseen analytical problems in mass spectrometer and hyphenated variants </li></ul>
<ul> <li> resolve and trouble-shoot practical issues with a mass spectrometer</li> <li> propose and optimise experimental conditions in hyphenated chromatographic-mass spectrometer analysis </li></ul>
<ul> <li> produce accurate, efficient and coherent reports on mass spectrometric and hyphenated measurements and their analysis</li> <li> participate in online discussions and tutorial sessions using enhanced communication skills </li></ul><div> </div><div> </div>
<div> This unit is delivered entirely via distance learning, including assessment. The course runs over 10 weeks, with a nominal 15h/week of student work. </div><div> </div><div> A typical week consists of: - An overview of the material, presenting the learning objectives for the week. - Explanatory material (~5h of student activity/week) in the form of video lectures, the course text and links to further online resources. </div><div> </div><div> Self-Study Exercises (~2h/week). These are formative, with feedback for them given in the tutorial. - Discussion (~1h/week). Students are encouraged to discuss the exercises and material in the forums where tutors will facilitate peer learning, providing feedback/input where necessary.</div><div> </div><div> Formative Assessment (~1h/week). This is to highlight misconceptions ready for the tutorial.</div><div> </div><div> Tutorial (~1h/week). Students will video conference with their tutor in groups of 6-8 to discuss and give/receive feedback. - Reflective journal (~1 h/week). Guided by questions related to weekly subject matter. </div><div> </div><div> There is also private study of ~4h/week consisting of: - Revision - Coursework e.g. critical analysis of research literature - Further practice (e.g. after the tutorials) - Independent/further study </div><div> </div><div> The times specified will vary greatly through the weeks. For example week 7 is mostly dedicated to the use of SIMION, Week 8 will concern data interpretation. Week 10 will involve more private study and summative assessment exercises. </div>
2
Written assignment (inc essay)
70%
7
Oral assessment/presentation
30%
<p>Online feedback on quizzes</p><p>Written feedback in written assignments/reports/journals</p><p>Group feedback and discussion</p>
CHEM63003 AS Online Conditions (Programme 09612)
N
<div> Quantitative Chemical Analysis (Ninth edition),Daniel C Harris & Charles A Lucy, Macmillan Education (2015), Available as E-book through UoM, ISBN: 9781319154141</div><div> </div><div> Introduction to mass spectrometry : instrumentation, applications and strategies for data interpretation / J. Throck Watson, O. David Sparkman. (4th ed.) Watson, J. Throck; Sparkman, O. David (Orrin David) John Wiley & Sons 2007 </div><div> </div><div> Mass spectrometry : principles and applications / Edmond de Hoffmann, Vincent Stroobant. (3rd edition.) Hoffmann, Edmond de; Stroobant, Vincent John Wiley & Sons 2007</div><div> </div><div> </div><div> A global view of LC/MS : how to solve your most challenging analytical problems / Ross Willoughby, Edward Sheehan, Samuel Mitrovich. (2nd edition.) Willoughby, Ross Clark; Sheehan, Edward (Edward W.); Mitrovich, Samuel Global View Publ. 2002 </div><div> </div><div> </div><div> MASS SPECTROMETRY FOR THE NOVICE BY JOHN; ROBOZ, JOHN GREAVES ISBN 10: 1420094181 </div><div> </div><div> Published papers from relevant peer-reviewed journals </div>
eAssessment
150
0
50